Laboratory taste and clinical examination of body organ systems

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Laboratory taste and clinical examination of body organ systems Laboratory techniques are the set of procedures used on natural sciences such as chemistry, biology, Therefore, safety precautions are vitally important.

Equipment and supplies

Three beakers, an Erlenmeyer flask, a graduated cylinder and a volumetric flask
Laboratory equipment refers to the various tools and equipment used by scientists working in a laboratory:

The classical equipment includes tools such as Bunsen burners and microscopes as well as specialty equipment such as operant conditioning chambers, spectrophotometers and calorimeters. Chemical laboratories
laboratory glassware such as the beaker or reagent bottle
Analytical devices as HPLC or spectrophotometers
Molecular biology laboratories + Life science laboratories
Autoclave
Microscope
Centrifuges
Shakers & mixers
Pipette
Thermal cyclers (PCR)
Photometer
Refrigerators and Freezers
Universal testing machine
ULT Freezers
Incubators
Bioreactor
Biological safety cabinets
Sequencing instruments
Fume hoods
Environmental chamber
Humidifier
Weighing scale
Reagents (supply)
Pipettes tips (supply)
Polymer (supply) consumables for small volumes (µL and mL scale), mainly sterile
Laboratory equipment is generally used to either perform an experiment or to take measurements and gather data. Larger or more sophisticated equipment is generally called a scientific instrument. Specialized types
The title of laboratory is also used for certain other facilities where the processes or equipment used are similar to those in scientific laboratories. These notably include:

Film laboratory or Darkroom
Clandestine lab for the production of illegal drugs
Computer lab
Crime lab used to process crime scene evidence
Language laboratory
Medical laboratory (involves handling of chemical compounds)
Public health laboratory
Industrial laboratory

Safety
Main article: Laboratory safety

An eyewash station in a laboratory. Geneticist Riin Tamm wearing protective lab coat
In many laboratories, hazards are present. Laboratory hazards might include poisons; infectious agents; flammable, explosive, or radioactive materials; moving machinery; extreme temperatures; lasers, strong magnetic fields or high voltage. Rules exist to minimize the individual's risk, and safety equipment is used to protect the lab users from injury or to assist in responding to an emergency. The Occupational Safety and Health Administration (OSHA) in the United States, recognizing the unique characteristics of the laboratory workplace, has tailored a standard for occupational exposure to hazardous chemicals in laboratories. This standard is often referred to as the "Laboratory Standard". Under this standard, a laboratory is required to produce a Chemical Hygiene Plan (CHP) which addresses the specific hazards found in its location, and its approach to them. In determining the proper Chemical Hygiene Plan for a particular business or laboratory, it is necessary to understand the requirements of the standard, evaluation of the current safety, health and environmental practices and assessment of the hazards. The CHP must be reviewed annually. Many schools and businesses employ safety, health, and environmental specialists, such as a Chemical Hygiene Officer (CHO) to develop, manage, and evaluate their CHP. Additionally, third party review is also used to provide an objective "outside view" which provides a fresh look at areas and problems that may be taken for granted or overlooked due to habit. Inspections and audits like also be conducted on a regular basis to assess hazards due to chemical handling and storage, electrical equipment, biohazards, hazardous waste management, chemical waste, housekeeping and emergency preparedness, radiation safety, ventilation as well as respiratory testing and indoor air quality. An important element of such audits is the review of regulatory compliance and the training of individuals who have access to or work in the laboratory. Training is critical to the ongoing safe operation of the laboratory facility. Educators, staff and management must be engaged in working to reduce the likelihood of accidents, injuries and potential litigation. Efforts are made to ensure laboratory safety videos are both relevant and engaging.[15]

Organization
Organization of laboratories is an area of focus in sociology. Scientists consider how their work should be organized, which could be based on themes, teams, projects or fields of expertise. Work is divided, not only between different jobs of the laboratory such as the researchers, engineers and technicians, but also in terms of autonomy (should the work be individual or in groups).[16] For example, one research group has a schedule where they conduct research on their own topic of interest for one day of the week, but for the rest they work on a given group project.[17] Finance management is yet another organizational issue. The laboratory itself is a historically dated organizational model. It came about due to the observation that the quality of work of researchers who collaborate is overall greater than a researcher working in isolation. From the 1950s, the laboratory has evolved from being an educational tool used by teachers to attract the top students into research, into an organizational model allowing a high level of scientific productivity. Some forms of organization in laboratories include:

Their size: Varies from a handful of researches to several hundred. The division of labor: "Occurs between designers and operatives; researchers, engineers, and technicians; theoreticians and experimenters; senior researchers, junior researchers and students; those who publish, those who sign the publications and the others; and between specialities." [18]
The coordination mechanisms: Which includes the formalization of objectives and tasks; the standardization of procedures (protocols, project management, quality management, knowledge management), the validation of publications and cross-cutting activities (number and type of seminars). There are three main factors that contribute to the organizational form of a laboratory :

The educational background of the researchers and their socialization process. The intellectual process involved in their work, including the type of investigation and equipment they use. The laboratory's history. Other forms of organization include social organization. Social organization
A study by Richard H.R. Harper, involving two laboratories, will help elucidate the concept of social organization in laboratories. The main subject of the study revolved around the relationship between the staff of a laboratory (researchers, administrators, receptionists, technicians, etc.) and their Locator. A Locator is an employee of a Laboratory who is in charge of knowing where each member of the laboratory currently is, based on a unique signal emitted from the badge of each staff member. The study describes social relationships among different classes of jobs, such as the relationship between researchers and the Locator. It does not describe the social relationship between employees within a class, such as the relationship between researchers. Through ethnographic studies, one finding is that, among the personnel, each class (researchers, administrators...) has a different degree of entitlement, which varies per laboratory. Entitlement can be both formal or informal (meaning it's not enforced), but each class is aware and conforms to its existence. The degree of entitlement, which is also referred to as a staff's rights, affects social interaction between staff. By looking at the various interactions among staff members, we can determine their social position in the organization. As an example, administrators, in one lab of the study, do not have the right to ask the Locator where the researchers currently are, as they are not entitled to such information. On the other hand, researchers do have access to this type of information. So a consequence of this social hierarchy is that the Locator discloses various degrees of information, based on the staff member and their rights. The Locator does not want to disclose information that could jeopardize his relationship with the members of staff. The Locator adheres to the rights of each class. Social hierarchy is also related to attitudes towards technologies. This was inferred based on the attitude of various jobs towards their lab badge. Their attitude depended on how that job viewed their badge from a standpoint of utility, (how is the badge useful for my job) morality (what are my morals on privacy, as it relates to being tracked by this badge) and relations (how will I be seen by others if I refuse to wear this badge). For example, a receptionist would view the badge as useful, as it would help them locate members of staff during the day. Illustrating relations, researchers would also wear their badge due to informal pressures, such as not wanting to look like a spoil-sport, or not wanting to draw attention to themselves. Another finding is the resistance to change in a social organization. Staff members feel ill at ease when changing patterns of entitlement, obligation, respect, informal and formal hierarchy, and more. In summary, differences in attitude among members of the laboratory are explained by social organization: A person's attitudes are intimately related to the role they have in an organization. This hierarchy helps understand information distribution, control, and attitudes towards technologies in the laboratory.[17]

A thorough assessment of the heart provides valuable information about the function of a patient’s cardiovascular system...
21/04/2023

A thorough assessment of the heart provides valuable information about the function of a patient’s cardiovascular system. Understanding how to properly assess the cardiovascular system and identifying both normal and abnormal assessment findings will allow the nurse to provide quality, safe care to the patient.

Before assessing a patient’s cardiovascular system, it is important to understand the various functions of the cardiovascular system. In addition to the information provided in the “Review of Cardiac Basics” section, the following images provide an overview of the cardiovascular system. provides an overview of the structure of the heart. Note the main cardiac structures are the atria, ventricles, and heart valves. demonstrates blood flow through the heart. Notice the flow of deoxygenated blood from the posterior and superior vena cava into the right atria and ventricle during diastole (indicated by the blue coloring of these structures). The right ventricle then pumps deoxygenated blood to the lungs via the pulmonary artery during systole. At the same time, oxygenated blood from the lungs returns to the left atria and ventricle via the pulmonary veins during diastole (indicated by the red coloring of these structures) and then is pumped out to the body via the aorta during systole. demonstrates the conduction system of the heart. This image depicts the conduction pathway through the heart as the tissue responds to electrical stimulation. illustrates the arteries of the circulatory system and depicts the veins of the circulatory system. The purpose of these figures is to facilitate an understanding of the electrical and mechanical function of the heart within the cardiovascular system.

Many different tests are used to diagnose heart disease. Besides blood tests and a chest X-ray, tests to diagnose heart ...
21/04/2023

Many different tests are used to diagnose heart disease. Besides blood tests and a chest X-ray, tests to diagnose heart disease can include:
Electrocardiogram (ECG or EKG). An ECG is a quick and painless test that records the electrical signals in the heart. It can tell if the heart is beating too fast or too slowly.
Holter monitoring. A Holter monitor is a portable ECG device that's worn for a day or more to record the heart's activity during daily activities. This test can detect irregular heartbeats that aren't found during a regular ECG exam.
Echocardiogram. This noninvasive exam uses sound waves to create detailed images of the heart in motion. It shows how blood moves through the heart and heart valves. An echocardiogram can help determine if a valve is narrowed or leaking.
Exercise tests or stress tests. These tests often involve walking on a treadmill or riding a stationary bike while the heart is monitored. Exercise tests help reveal how the heart responds to physical activity and whether heart disease symptoms occur during exercise. If you can't exercise, you might be given medications.
Cardiac catheterization. This test can show blockages in the heart arteries. A long, thin flexible tube (catheter) is inserted in a blood vessel, usually in the groin or wrist, and guided to the heart. Dye flows through the catheter to arteries in the heart. The dye helps the arteries show up more clearly on X-ray images taken during the test.
Heart (cardiac) CT scan. In a cardiac CT scan, you lie on a table inside a doughnut-shaped machine. An X-ray tube inside the machine rotates around your body and collects images of your heart and chest.
Heart (cardiac) magnetic resonance imaging (MRI) scan. A cardiac MRI uses a magnetic field and computer-generated radio waves to create detailed images of the heart.

To diagnose the respiratory failure, your doctor may order some of the following tests and procedures.Arterial blood gas...
21/04/2023

To diagnose the respiratory failure, your doctor may order some of the following tests and procedures.
Arterial blood gas tests measure levels of oxygen, carbon dioxide, pH, and bicarbonate. A sample of your blood will be taken from your arteries. These tests help determine whether you have respiratory failure and what type it is.
Blood tests can help find the cause of your respiratory failure. Blood tests can also help your doctor see how well your other organs are working.
Bacterial cultures can show a bacterial infection in samples of your blood, urine, or phlegm (a slimy substance that you cough out).
Bronchoscopy can show blockages, tumors, or other possible causes of respiratory failure.
Lung imaging tests show detailed images of the lungs and can reveal any possible inflammation or damage.
Heart tests such as electrocardiograms and echocardiography show how well your heart is working.
A lung biopsy shows changes in the cells of your lung tissue or tiny germs or microbes causing infections.
Lung function tests measure how well your lungs are working.
Chronic obstructive pulmonary disease (COPD) is a long-term lung disease.

Most devices for self-testing (home testing) are modified versions of professional tests, with comparable sensitivity an...
04/12/2022

Most devices for self-testing (home testing) are modified versions of professional tests, with comparable sensitivity and specificity.
Self-tests are usually reliable for long-standing infections, but are sometimes unable to detect recent HIV infections acquired in the past few weeks.
Like any screening test, a reactive (‘positive’) result must be confirmed with one or two follow-up tests.
Studies show that most people can accurately and reliably use self-testing devices.
HIV self-testing involves collecting your own specimen (oral fluid or blood) and using a rapid HIV test kit. You perform the whole test yourself, including reading and interpreting the test result.
This is different from HIV self-sampling. With self-sampling, you collect your own sample and send it to a laboratory for analysis. We have another page on the accuracy of self-sampling.
Self-testing is sometimes called ‘home testing’, because people often do it at home. The World Health Organization (WHO) recommends that HIV self-testing should be offered alongside existing HIV testing services.
Different types of tests
The latest news and research on self-testing & home testing
Self-tests are usually modified versions of rapid, point-of-care test kits that were originally designed for healthcare professionals. Their processes, packaging and instructions have been simplified so as to guide you through the steps of taking a test.
With some tests, you use a lancet to release a small quantity of blood from a fingerprick. Other tests require a sample of oral fluid, obtained by swabbing an absorbent pad around the outer gums, adjacent to the teeth.
Self-tests may be second- or third-generation HIV tests. They are only able to detect HIV antibodies, whereas the fourth-generation HIV tests which are normally used by healthcare professionals are also able to detect p24 antigen (a protein contained in HIV's viral core that can be detected in the first few weeks after infection). Fourth-generation tests are therefore better at picking up recent infections.
Find out more: Home HIV testing (self-testing and self-sampling)
Second- and third-generation tests can accurately detect chronic (long standing) HIV infection. But their ability to detect recently acquired HIV is more variable.
Their window periods are a little longer than for other tests. The window period is the time immediately after infection when tests may not detect markers of infection and therefore give a false negative result.
Across the world, the majority of HIV self-tests are based on second-generation testing technology. They can detect immunoglobulin G (IgG) antibodies, but not immunoglobulin M (IgM) antibodies or p24 viral antigen. As these two substances are detectable sooner after HIV infection than IgG antibodies, second-generation tests have longer window periods. The OraQuick In-Home HIV Test, SURE CHECK HIV Self Test and autotest VIH are CE-marked second-generation self-tests.
The SURE CHECK HIV Self Test and autotest VIH are both adapted from a device that is widely used by health professionals. This may be marketed either as the Chembio Sure Check HIV 1/2 Assay or as the Clearview Complete HIV 1/2 Assay. In relation to use by health professionals, American regulators assessed this assay’s sensitivity (proportion of HIV-positive samples accurately described as such) to be 99.7% and its specificity (proportion of HIV-negative samples accurately described as such) to be 99.9%. In relation to the two self-tests, studies to assess the ability of lay users to follow instructions, use the test and correctly interpret its results found that 97-98% achieved a valid result.
self-testing
antibody
oral
sensitivity
oral fluid
Other HIV self-tests are based on third-generation testing technology. They can detect immunoglobulin G (IgG) antibodies and immunoglobulin M (IgM) antibodies, therefore shortening the window period. The INSTI HIV Self Test, Exacto Test HIV and atomo HIV Self Test are CE-marked third-generation tests. In some countries, the atomo device is repackaged and marketed as the Mylan HIV Self Test or as the Simplitude ByMe HIV Self Test.
The INSTI HIV Self Test is adapted from the INSTI HIV-1/HIV-2 Antibody Test for personal use. This is one of the most accurate third-generation tests available, with a superior sensitivity to recent infection. When used by health professionals, the test’s sensitivity and specificity were both calculated to be 99.8%.
Are people able to understand test instructions and use self-tests reliably?
A review identified 25 studies in which people used self-tests, with their results compared with those of a healthcare worker testing them at the same time. The original studies were done in a mix of North American, African, European and Asian countries. Fifteen studies used oral fluid-based tests, six used blood-based tests and four used both oral and blood specimens. Of note, several studies were evaluating professional assays that had not been adapted for use as self-tests – performance could be better in commercially marketed tests.
Basic information on testing
The researchers found that most people could reliably and accurately use rapid tests. Using a statistical approach known as Cohen’s kappa in which 1 represents perfect agreement between the results of a self-tester and a trained health worker and less than 1 represents less than perfect agreement, results were highly concordant. The Cohen’s kappa was 0.98 in studies in which users first received a demonstration of how to do the self-test or to interpret its result. In studies in which this was not provided, it was 0.97. This means that in only a small minority of cases the self-tester and the healthcare worker got results that were different from each other.
There were more invalid results in people using blood-based tests (0.4-9.5%) than studies using oral fluid-based tests (0.2-4.5%). Common errors included incorrect or incomplete specimen collection, spilling or incorrect use of the buffer solution, problems transferring blood samples, and difficulties with the interpretation of results.

Although blood-based rapid diagnostic tests might have the potential to deliver more accurate results, more invalid results might occur because the greatest number of user errors was related to standard procedures when capillary tubes and pipettes were used. Simpler test procedures and clearer instructions might mitigate these problems.

Situations in which self-tests may not be accurate
Performance of self-tests is poorer in a number of situations. Results may not be accurate.
In cases of recent HIV infection, during the test’s window period.
In people with diagnosed HIV who are taking HIV treatment. These tests are not a reliable way to confirm that you still have HIV infection.
In people who are taking pre-exposure prophylaxis (PrEP) or post-exposure prophylaxis (PEP). If they acquire HIV, they may have a delayed antibody response, extending the window period.
When test instructions have not been correctly followed – for example not enough blood has been collected.

What is an HIV self-test?An HIV self-test (or rapid self-test) is an antibody test that can be used at home or in a priv...
04/12/2022

What is an HIV self-test?
An HIV self-test (or rapid self-test) is an antibody test that can be used at home or in a private location. With an HIV self-test, you can get your test results within 20 minutes.

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