03/20/2026
Thank you for participating in Under the Scope with Octavius, who celebrated histologists this week for Histotechnology Professionals Day (March 10)! This week's answer is...
Glomerular basement membrane stained with periodic acid-Schiff (PAS) and counterstained with hematoxylin.
PAS staining reacts with carbohydrate-rich structures, making components such as the basement membrane and mesangial matrix appear vivid magenta. The hematoxylin counterstain provides contrast by coloring cell nuclei deep blue to purple, helping distinguish cellular structures from the surrounding tissue.