Oroboros Instruments

Oroboros Instruments Oroboros Instruments distributes the gold standard for O2k-technology High-Resolution Respirometry. We are a scientifically oriented organization.

New publication highlight: mtG3PDH inhibition in androgen receptor-negative prostate cancer cells shows a strong depende...
05/02/2026

New publication highlight: mtG3PDH inhibition in androgen receptor-negative prostate cancer cells shows a strong dependence on extracellular pyruvate concentrations, affecting routine respiration and H₂O₂ production. A good reminder that the same enzyme inhibition can look different depending on substrate context and assay design.

The Gp-pathway is often underused in pathway-resolved bioenergetics. mtG3PDH oxidizes glycerophosphate and feeds electro...
03/02/2026

The Gp-pathway is often underused in pathway-resolved bioenergetics. mtG3PDH oxidizes glycerophosphate and feeds electrons into the Q-junction, and SUIT protocols can quantify this contribution directly. If you care about ET-pathway partitioning, it’s a powerful handle.

Pyruvate isn’t always a “neutral” background substrate. If you use pyruvate in the protocol, keep in mind it can competi...
02/02/2026

Pyruvate isn’t always a “neutral” background substrate. If you use pyruvate in the protocol, keep in mind it can competitively reverse cyanide inhibition of CIV, making CIV inhibition less reliable. For robust CIV blockages, azide may be the better choice under pyruvate-containing conditions.

Small handling detail, big impact on O₂ control.When adding permeabilized fibers to the Oroboros  chamber, pause stirrin...
29/01/2026

Small handling detail, big impact on O₂ control.
When adding permeabilized fibers to the Oroboros chamber, pause stirring while the chamber is open and restart only when it’s nearly closed. This helps minimize changes in O₂ concentration during sample addition and keeps you closer to your intended O₂ setpoint.

Publication spotlight: Miller et al. (2017) examined mitochondrial respiration in highly aerobic Alaskan huskies in the ...
28/01/2026

Publication spotlight: Miller et al. (2017) examined mitochondrial respiration in highly aerobic Alaskan huskies in the non- raced state and after a 1600 km sled dog race (Iditarod). A striking model for endurance adaptation and mitochondrial plasticity under extreme energetic demand.

This week, coauthors Laner Verena, Gnaiger Erich, and Davis Michael S are back in Alaska – for an exciting workshop assessing not only respiratory control but also H2O2 production in muscle mitochondria of huskies.

DOI: 10.1371/journal.pone.0174874

🧪🐾 Respiratory control & H₂O₂ in elite endurance mitochondria, in Alaska.At the 179th Oroboros Workshop on High-Resoluti...
26/01/2026

🧪🐾 Respiratory control & H₂O₂ in elite endurance mitochondria, in Alaska.
At the 179th Oroboros Workshop on High-Resolution Respirometry (Jan 26–30, 2026 | Big Lake, AK, US) we’ll combine a small set of intro scientific lectures with hands-on research in comparative mitochondrial physiology. The scientific focus is very concrete: respiratory control and hydrogen peroxide production as a function of oxygen concentration, with guided data analysis, discussion, and interpretation.

What makes this workshop special is the model system: skeletal muscle from racing Alaskan Husky sled dogs, alongside the chance to see the “real-world phenotype” up close. Participants will not only work in the lab (permeabilized fibers vs isolated mitochondria on the Oroboros Bioenergetics Platform) but will also be out with the dogs, learning how a racing team operates and helping drive sleds under professional supervision.

📍 Hosted at the Oklahoma State University Comparative Exercise Physiology Laboratory, on the grounds of Happy Trails Kennel (Big Lake, AK). The kennel is run by Martin Buser, a 4-time Iditarod winner, and this workshop builds on the earlier Big Lake collaboration where canine skeletal muscle HRR work helped seed a published study (Miller et al., 2017, PLOS ONE).
Details: https://buff.ly/5RZYzPp

From chaos to clarity: harmonization in mitochondrial studies requires shared language and standardized workflows. Consi...
23/01/2026

From chaos to clarity: harmonization in mitochondrial studies requires shared language and standardized workflows. Consistent terminology and procedures reduce ambiguity, prevent conflicting standards, and make datasets genuinely comparable across labs, timepoints, and cohorts.

Adding superoxide dismutase (SOD) in the Amplex UltraRed assay is more than a conversion step to generate H₂O₂ from  O₂⁻...
21/01/2026

Adding superoxide dismutase (SOD) in the Amplex UltraRed assay is more than a conversion step to generate H₂O₂ from O₂⁻•. It also reduces chemical background from NADH or glutathione auto oxidation and prevents horseradish peroxidase (HRP) inhibition by superoxide. Net effect: more trustworthy H₂O₂ flux estimates.

Publication spotlight: Mueller et al. (2025) show that among ectothermic vertebrates with different standard metabolic r...
19/01/2026

Publication spotlight: Mueller et al. (2025) show that among ectothermic vertebrates with different standard metabolic rates, lizards have the highest mitochondrial ATP production capacity and OXPHOS coupling efficiency. Furthermore, the authors propose that mitochondrial ROS production in lizards is limited by a facilitated proton leak mechanism, highlighting different metabolic strategies among clades.

Mueller et al. (2025), Proc R Soc B. DOI: 10.1098/rspb.2025.0374

How to normalize when comparing mitochondrial function across samples that might differ in their mitochondrial content: ...
15/01/2026

How to normalize when comparing mitochondrial function across samples that might differ in their mitochondrial content:

Scaling oxygen flux to a mitochondrial marker such as citrate synthase (CS) activity accounts for differences in mitochondrial density, and the marker specific oxygen flux (JO₂/CS) reflects mitochondrial quality rather than quantity.

Hidden pitfall in Amplex UltraRed assays: In tissues like liver, kidney, intestine, or stomach, mitochondrial carboxyles...
14/01/2026

Hidden pitfall in Amplex UltraRed assays:

In tissues like liver, kidney, intestine, or stomach, mitochondrial carboxylesterases can oxidize AmR without H₂O₂ (and without HRP), generating a misleading fluorescence slope.

Adding PMSF to the assay medium helps shut down this artefact and keeps H₂O₂ flux interpretation on track.

Publication spotlight: GRACILE syndrome is a severe mitochondrial disorder caused by mutations leading to Complex III de...
12/01/2026

Publication spotlight: GRACILE syndrome is a severe mitochondrial disorder caused by mutations leading to Complex III deficiency.

In a GRACILE model, mitochondrial H₂O₂ production was elevated in kidney and heart and was reduced by alternative oxidase AOX expression. While increased H₂O₂ was excluded as an early pathogenesis mechanism, the data support the idea that ROS can still shape late -stage disease trajectories.

Rajendran et al., EMBO Mol Med (2019). DOI: 10.15252/emmm.201809456

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