Oroboros Instruments

Oroboros Instruments Oroboros Instruments distributes the gold standard for O2k-technology High-Resolution Respirometry. We are a scientifically oriented organization.

06/11/2025

Praying for Paolo, Marco, and their Nepali friend Padam Tamang

Our team member Paolo, former team member Marco, and Padam Tamang, a Sherpa mountain guide from the Rolwaling Team (Dreamers Destination) were caught in an avalanche during their “The Mountain of Light Expedition 2025” in Nepal last Monday. Paolo tragically lost his life, and Marco and Padam still remain missing even on the fourth day after the accident.

The news about the avalanche tragedy remains incomprehensible to all of us. We remember them as special persons who helped us to climb higher. Our thoughts are with their families and friends in this terrible time.

How to measure mitochondrial membrane potential with the Oroboros instrument Oroboros supports two established approache...
05/11/2025

How to measure mitochondrial membrane potential with the Oroboros instrument

Oroboros supports two established approaches, simultaneously with respiration:

Fluorometric (Fluo-Module):
Use fluorophores such as TMRM, safranin O, or rhodamine 123 to track membrane potential-dependent changes during HRR with multiple titrations.

Potentiometric (TPP⁺ ISE-Module):
Monitor TPP⁺ distribution with the ion-selective electrode and quantify the membrane potential.
This provides a direct, concentration-based readout independent of optical path or dye quenching.

What we call “normoxia” in the lab is often hyperoxia for cells and mitochondria. To interpret respiratory data correctl...
03/11/2025

What we call “normoxia” in the lab is often hyperoxia for cells and mitochondria. To interpret respiratory data correctly, oxygen must be carefully set, monitored, and reported. Read the perspective that clarifies hypoxia/normoxia/hyperoxia for mitochondrial studies:

Donnelly et al. (2022) The ABC of hypoxia—what is the norm. Bioenerg Commun 2022.12.v2. https://buff.ly/pvY1L0n

DatLab Tip: Prevent missing data with experiment-specific “Lab layouts.”Adapt a standard layout to the requirements of y...
31/10/2025

DatLab Tip: Prevent missing data with experiment-specific “Lab layouts.”

Adapt a standard layout to the requirements of your experiment and save it as a Lab layout. All saved layouts are listed under Layouts, where you (and your team) can rename, export, import, or delete them—keeping project-specific configurations organized and reusable.

Why this matters
• Consistent trace visualization
• Reduced operator error and misinterpretation of data
• Reusable, shareable configurations for standardized analyses

Structured layouts → more efficient review and interpretation.

Can fluorescent probes interfere with mitochondrial respiration? Yes. Several fluorescent dyes—and, to a lesser extent, ...
29/10/2025

Can fluorescent probes interfere with mitochondrial respiration?

Yes. Several fluorescent dyes—and, to a lesser extent, TPP⁺—can inhibit or uncouple respiration. The effect depends on the probe, concentration, and sample type.

Best practice: validate respirometrically before using a new probe or changing tissue/state.

We are proud to contribute at the 17th Conference of the Asian Society of Mitochondrial Research and Medicine (ASMRM2025...
27/10/2025

We are proud to contribute at the 17th Conference of the Asian Society of Mitochondrial Research and Medicine (ASMRM2025) in Yiwu, China.
Erich Gnaiger, presented: “Redox regulation of coenzyme-Q, mitochondrial membrane potential, and calcium uptake under hypoxia.”
Thank you to the organizers and participants for the excellent discussions.

DatLab 8 — Event Intervals Define and display the time between events in your SUIT or instrumental protocol. Use our sta...
22/10/2025

DatLab 8 — Event Intervals

Define and display the time between events in your SUIT or instrumental protocol. Use our standard interval programs or create your own to simplify execution and improve standardization and reproducibility.

https://buff.ly/MEUTc3F

Targeting mitochondrial coupling, a potential cancer therapy? Zunica et al. (2021, Cancer Metabolism) report that the mi...
21/10/2025

Targeting mitochondrial coupling, a potential cancer therapy?
Zunica et al. (2021, Cancer Metabolism) report that the mitochondrial uncoupler BAM15 suppresses proliferation and survival of aggressive breast cancer cells, pointing to uncoupling as a potential therapeutic avenue against metabolically adaptable tumors.

Citation: Zunica ERM, Axelrod CL, Cho E, et al. (2021). Cancer Metab 9:36.
https://buff.ly/mCkX9j5

Takeaway: The metabolic vulnerabilities of highly proliferative breast cancers may be leveraged to improve responses to existing therapies

A heartfelt thank you to everyone who attended the opening of our new Oroboros MitoAnalytics Laboratory! 🎉 Your support...
17/10/2025

A heartfelt thank you to everyone who attended the opening of our new Oroboros MitoAnalytics Laboratory! 🎉

Your support energizes our mission to deliver high-resolution mitochondrial testing, from PBMC assessments to pharmacological studies, complemented with expert reporting and clinical interpretation through our local partner.

We look forward to our next steps: more collaborations, more data, and greater impact.

See more at: https://buff.ly/Ikg8SdC

Warburg vs. Crabtree Warburg and Crabtree described metabolic reprogramming that modifies cellular energy production and...
16/10/2025

Warburg vs. Crabtree

Warburg and Crabtree described metabolic reprogramming that modifies cellular energy production and alters mitochondrial respiration through different mechanisms.

• Crabtree effect: classically in yeast, but also observed in mammalian cells, including cancer cells.

• Warburg effect: stimulation of aerobic glycolysis is a cancer hallmark, yet not consistent across all tumor types.

Can I measure mitochondrial membrane potential (mtMP) in all sample types? Not necessarily. Dye uptake and intracellular...
14/10/2025

Can I measure mitochondrial membrane potential (mtMP) in all sample types? Not necessarily.

Dye uptake and intracellular distribution can be limited by intact plasma membranes, meaning mtMP readouts in living cells may misrepresent the true mitochondrial membrane potential. The practical takeaway: perform rigorous pilot experiments to verify assay suitability for your preparation before drawing conclusions.

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