Oroboros Instruments

Oroboros Instruments Oroboros Instruments distributes the gold standard for O2k-technology High-Resolution Respirometry. We are a scientifically oriented organization.

How do oxygen levels and the choice of respiration medium influence our mitochondrial readouts? Doerrier et al. (2024, F...
27/11/2025

How do oxygen levels and the choice of respiration medium influence our mitochondrial readouts?

Doerrier et al. (2024, Free Radic Biol Med) show that high O₂ regimes (~250–400 µM) can markedly increase measured O₂ flux, and that these effects depend strongly on the respiration medium.

learn more: https://buff.ly/fOYOCyo

Air and zero calibration are essential quality control procedures. They not only ensure the proper functioning of the se...
24/11/2025

Air and zero calibration are essential quality control procedures. They not only ensure the proper functioning of the sensor, but also that the measured signal (in µA) is correctly converted into an O2 concentration (in µM) as a function of barometric pressure, experimental temperature and solubility factor of the medium. Inaccurate calibration will directly result in incorrect O₂ concentration values.

Following exercise, natural killer (NK) cells showed greater ability to kill triple-negative breast cancer (TNBC) cells ...
19/11/2025

Following exercise, natural killer (NK) cells showed greater ability to kill triple-negative breast cancer (TNBC) cells when tested under low-oxygen (hypoxic) conditions—reflecting the TNBC microenvironment. Under normoxic conditions, mitochondrial oxidative phosphorylation (OXPHOS) capacity of TNBC-activated NK cells was also elevated in the post-exercise state.

Several chemicals—e.g., colored substances such as cytochrome c—can interfere with the fluorescence signal when injected...
17/11/2025

Several chemicals—e.g., colored substances such as cytochrome c—can interfere with the fluorescence signal when injected into the Oroboros chamber. To evaluate potential interference, test the reagents in a background run without biological samples.

A warm welcome to Razita, our new Sales Butterfly. We are happy to have her as part of our Sales Athletes and to further...
13/11/2025

A warm welcome to Razita, our new Sales Butterfly. We are happy to have her as part of our Sales Athletes and to further strengthen the relationship with our valued customers.

In memory of Paolo Cocco, Marco Di Marcello and Padam Tamang.It is with deep sadness that we share news of the avalanche...
11/11/2025

In memory of Paolo Cocco, Marco Di Marcello and Padam Tamang.

It is with deep sadness that we share news of the avalanche tragedy in Rolwaling, Nepal. On 3rd November 2025, a massive avalanche hit the Dreamers Destination expedition team while they were climbing just below the summit of Yalung Ri (5,630 m).

Paolo Cocco, a graphic designer, IT specialist and photographer who worked with Oroboros Instruments in Innsbruck, could only be recovered dead by expedition leader Phurba Tsering Sherpa. Marco Di Marcello, a mountain guide with Italian and Canadian nationality, and Nepalese guide Padam Tamang are still missing. Due to adverse weather conditions, the search was officially called off on 7th November 2025.

Paolo and Marco had strong connections to the Umbrüggler Alm above Innsbruck — Marco had also worked there before joining Oroboros. On 9 November, colleagues and friends hiked to the Alm to honour Paolo and Marco and to grieve together.
Our hearts go out to their families, friends and the Rolwaling community.

Automated chamber O₂ control with TIP2k Use TIP2k to keep chamber oxygen within a defined experimental range. When worki...
10/11/2025

Automated chamber O₂ control with TIP2k

Use TIP2k to keep chamber oxygen within a defined experimental range. When working in MiR06 (MiR05 plus catalase), chamber reoxygenation can be achieved with H₂O₂. In feedback control mode, TIP2k regulates H₂O₂ injection so that O₂ remains within well-defined limits.

06/11/2025

Praying for Paolo, Marco, and their Nepali friend Padam Tamang

Our team member Paolo, former team member Marco, and Padam Tamang, a Sherpa mountain guide from the Rolwaling Team (Dreamers Destination) were caught in an avalanche during their “The Mountain of Light Expedition 2025” in Nepal last Monday. Paolo tragically lost his life, and Marco and Padam still remain missing even on the fourth day after the accident.

The news about the avalanche tragedy remains incomprehensible to all of us. We remember them as special persons who helped us to climb higher. Our thoughts are with their families and friends in this terrible time.

How to measure mitochondrial membrane potential with the Oroboros instrument Oroboros supports two established approache...
05/11/2025

How to measure mitochondrial membrane potential with the Oroboros instrument

Oroboros supports two established approaches, simultaneously with respiration:

Fluorometric (Fluo-Module):
Use fluorophores such as TMRM, safranin O, or rhodamine 123 to track membrane potential-dependent changes during HRR with multiple titrations.

Potentiometric (TPP⁺ ISE-Module):
Monitor TPP⁺ distribution with the ion-selective electrode and quantify the membrane potential.
This provides a direct, concentration-based readout independent of optical path or dye quenching.

What we call “normoxia” in the lab is often hyperoxia for cells and mitochondria. To interpret respiratory data correctl...
03/11/2025

What we call “normoxia” in the lab is often hyperoxia for cells and mitochondria. To interpret respiratory data correctly, oxygen must be carefully set, monitored, and reported. Read the perspective that clarifies hypoxia/normoxia/hyperoxia for mitochondrial studies:

Donnelly et al. (2022) The ABC of hypoxia—what is the norm. Bioenerg Commun 2022.12.v2. https://buff.ly/pvY1L0n

DatLab Tip: Prevent missing data with experiment-specific “Lab layouts.”Adapt a standard layout to the requirements of y...
31/10/2025

DatLab Tip: Prevent missing data with experiment-specific “Lab layouts.”

Adapt a standard layout to the requirements of your experiment and save it as a Lab layout. All saved layouts are listed under Layouts, where you (and your team) can rename, export, import, or delete them—keeping project-specific configurations organized and reusable.

Why this matters
• Consistent trace visualization
• Reduced operator error and misinterpretation of data
• Reusable, shareable configurations for standardized analyses

Structured layouts → more efficient review and interpretation.

Can fluorescent probes interfere with mitochondrial respiration? Yes. Several fluorescent dyes—and, to a lesser extent, ...
29/10/2025

Can fluorescent probes interfere with mitochondrial respiration?

Yes. Several fluorescent dyes—and, to a lesser extent, TPP⁺—can inhibit or uncouple respiration. The effect depends on the probe, concentration, and sample type.

Best practice: validate respirometrically before using a new probe or changing tissue/state.

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Schoepfstrasse 18
Innsbruck
6020

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