BMB

04/12/2025

برنامج تدريبي داخل - جامعة القاهرة لطلاب من مختلف الجامعات الحكومية والخاصة والأهلية..
عميد الكلية: البرنامج ترجمة حقيقية لجهود الدولة في تنفيذ استراتيجية التعليم العالي ورؤية مصر ٢٠٣٠، التي تهدف إلى بناء كوادر علمية متميزة، وتعزيز تبادل الخبرات بين الجامعات، ورفع كفاءة التدريب والبحث العلمي بما يواكب التطورات العالمية.
شهد السيد الأستاذ الدكتور/ أحمد سمير، عميد الكلية حفل ختام فعاليات البرنامج التدريبي المُقام بقسم الكيمياء الحيوية بالتعاون مع مركز بحوث وخدمات التكنولوجيا الحيوية بالكلية، بعنوان:
"اكتشاف وتطوير الأدوية باستخدام جهاز الفصل الكروماتوجرافي السائل عالي الأداء (HPLC)"
والذي جاء تعزيزًا لدور الكلية في تطوير المهارات البحثية والعملية للطلاب والباحثين في مجالات الكيمياء الحيوية، والتقنيات الجزيئية الحديثة.
وقد حضر البرنامج التدريبي نخبة من طلاب الجامعات المصرية المختلفة، من بينها:
جامعة القاهرة (كليات الطب البيطري – الزراعة – العلوم)، جامعة مصر للعلوم والتكنولوجيا (كلية البيوتكنولوجي)، جامعة أكتوبر للعلوم الحديثة والآداب MSA (كليات الصيدلة – البيوتكنولوجي)، وكليات الطب البيطري في جامعة بدر بالقاهرة، جامعة بنها، جامعة الجلالة
والجامعة المصرية الصينية،
جامعة الملك سلمان الدولية (كليات العلوم – الطب بيطري)، وكذلك جامعة النيل وجامعة العلمين الدولية وجامعة المنوفية الأهلية
وجامعة الزقازيق.
هذا التنوع الكبير في المشاركين يعكس الثقة الأكاديمية في البرنامج التدريبي وقدرته على تقديم محتوى علمي عملي عالي الجودة.
هذا وقد قام السيد عميد الكلية بتسليم شهادات اجتياز الدورة للمتدربين، وذلك بحضور:
– السيدة الأستاذ الدكتور/ إيمان معوض، رئيس قسم الكيمياء الحيوية والبيولوجيا الجزيئية
– السيد الدكتور/ أحمد عرابي، نائب مدير مركز بحوث وخدمات التكنولوجيا الحيوية
جدير بالذكر أن تنسيق وتنظيم الدورة التدريبية كان تحت إشراف
السيد الأستاذ الدكتور/ عبدالباري برنس،
أستاذ التكنولوجيا الحيوية – كلية الطب البيطري جامعة القاهرة
والذي قام بالإعداد العلمي والتنسيق والتواصل مع المشاركين لضمان أعلى مستوى من الجودة التدريبية.
هذا وقد أشاد المشاركون بالمحتوى العلمي للبرنامج التدريبي كما أكدوا أن التدريب قدم لهم خبرات عملية متميزة ودعمًا حقيقيًا لمسيرتهم العلمية.
ومن جانبه أعرب الدكتور/ أحمد سمير، عميد الكلية عن سعادته بهذا العمل الذي تجمع فيه طلاب من مختلف جامعات مصر، مما يعزز مكانة كلية الطب البيطري – جامعة القاهرة كصرح رائد في التدريب والبحث العلمي تماشيا مع استراتيجية التعليم العالي في مصر المنبثقة من استراتيجية مصر ٢٠٣٠، كما توجه بخالص الشكر للسيدة أ.د/ إيمان معوض، رئيس قسم الكيمياء والسيد أ.د/ عبد الباري برنس، منسق البرنامج التدريبي، والسد د/ أحمد عرابي، نائب مدير مركز بحوث البيوتكنولوجي بالكلية.


A training program at the Faculty of Veterinary Medicine – Cairo University, offered to students from various public, private, and national universities.
Dean of the Faculty: The program represents a true reflection of the state’s efforts to implement the Higher Education Strategy and Egypt’s Vision 2030, which aim to build distinguished scientific competencies, enhance the exchange of expertise among universities, and raise the efficiency of training and scientific research in line with global developments.
Prof. Ahmed Samir, Dean of the Faculty, attended the closing ceremony of the training program held at the Department of Biochemistry in cooperation with the Biotechnology Research and Services Center, entitled:
“Drug Discovery and Development Using High-Performance Liquid Chromatography (HPLC)”.
This program comes as part of the Faculty’s commitment to enhancing the research and practical skills of students and researchers in the fields of biochemistry and modern molecular technologies.
The training program was attended by an elite group of students from various Egyptian universities, including:
Cairo University (Faculties of Veterinary Medicine – Agriculture – Science), Misr University for Science and Technology (Faculty of Biotechnology), October University for Modern Sciences and Arts – MSA (Faculties of Pharmacy – Biotechnology), as well as students from the Faculties of Veterinary Medicine at Badr University in Cairo, Benha University, Galala University, the Egyptian Chinese University, King Salman International University (Faculties of Science – Veterinary Medicine), in addition to Nile University, Alamein International University, Menoufia National University, and Zagazig University.
This wide diversity of participants reflects the academic confidence in the program and its ability to provide high-quality scientific and practical content.
The Dean presented the certificates of completion to the trainees, in the presence of:
– Prof. Eman Moawad, Head of the Department of Biochemistry and Molecular Biology
– Dr. Ahmed Orabi, Deputy Director of the Biotechnology Research and Services Center
The training program was coordinated and supervised by Prof. Abdel-Bary Prince, Professor of Biotechnology – Faculty of Veterinary Medicine, Cairo University, who oversaw the scientific preparation and communication with participants to ensure the highest standards of training quality.
Participants praised the scientific content of the program, confirming that the training provided them with distinguished practical experience and real support for their academic and research careers.
For his part, Prof. Ahmed Samir, Dean of the Faculty, expressed his happiness with this event, which brought together students from various Egyptian universities, reinforcing the leading role of the Faculty of Veterinary Medicine – Cairo University as a distinguished center in training and scientific research.
This comes in line with Egypt’s Higher Education Strategy derived from Egypt Vision 2030.
He also extended his sincere thanks to Prof. Iman Moawad, Prof. Abdel-Bary Prince – Training Program Coordinator, and Dr. Ahmed Orabi – Deputy Director of the Biotechnology Research Center.

02/06/2023

20/03/2019

MTT Tetrazolium Assay Concept
The MTT 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide tetrazolium reduction assay was the first homogeneous cell viability assay developed for a 96-well format that was suitable for high throughput screening HTS . The MTT tetrazolium assay technology has been widely adopted and remains popular in academic labs as evidenced by thousands of published articles. The MTT substrate is prepared in a physiologically balanced solution, added to cells in culture, usually at a final concentration of 0.2 - 0.5mg/ml, and incubated for 1 to 4 hours. The quantity of formazan presumably directly proportional to the number of viable cells is measured by recording changes in absorbance at 570 nm using a plate reading spectrophotometer. A reference wavelength of 630 nm is sometimes used, but not necessary for most assay conditions. Viable cells with active metabolism convert MTT into a purple colored formazan product with an absorbance maximum near 570 nm 1 . When cells die, they lose the ability to convert MTT into formazan, thus color formation serves as a useful and convenient marker of only the viable cells. The exact cellular mechanism of MTT reduction into formazan is not well understood, but likely involves reaction with NADH or similar reducing molecules that transfer electrons to MTT. Speculation in the early literature involving specific mitochondrial enzymes has led to the assumption mentioned in numerous publications that MTT is measuring mitochondrial activity. The formazan product of the MTT tetrazolium accumulates as an insoluble precipitate inside cells as well as being deposited near the cell surface and in the culture medium. The pH of the solubilization solution can be adjusted to provide maximum absorbance if sensitivity is an issue; however, other assay technologies offer much greater sensitivity than MTT. The amount of signal generated is dependent on several parameters including: the concentration of MTT, the length of the incubation period, the number of viable cells and their metabolic activity. All of these parameters should be considered when optimizing the assay conditions to generate a sufficient amount of product that can be detected above background. The conversion of MTT to formazan by cells in culture is time dependent. Longer incubation time will result in accumulation of color and increased sensitivity up to a point; however, the incubation time is limited because of the cytotoxic nature of the detection reagents which utilize energy reducing equivalents such as NADH from the cell to generate a signal. For cell populations in log phase growth, the amount of formazan product is generally proportional to the number of metabolically active viable cells as demonstrated by the linearity of response. Culture conditions that alter the metabolism of the cells will likely affect the rate of MTT reduction into formazan. For example, when adherent cells in culture approach confluence and growth becomes contact inhibited, metabolism may slow down and the amount MTT reduction per cell will be lower. That situation will lead to a loss of linearity between absorbance and cell number. Other adverse culture conditions such as altered pH or depletion of essential nutrients such as glucose may lead to a change in the ability of cells to reduce MTT. The MTT assay was developed as a non-radioactive alternative to tritiated thymidine incorporation into DNA for measuring cell proliferation. In many experimental situations, the MTT assay can directly substitute for the tritiated thymidine incorporation assay. However, it is worth noting that MTT reduction is a marker reflecting viable cell metabolism and not specifically cell proliferation. Tetrazolium reduction assays are oft enerroneously described as measuring cell proliferation without the use of proper controls to confirm effects on metabolism. Shortly after addition of MTT, the morphology of some cell types can be observed to change dramatically suggesting altered physiology. Toxicity of the MTT compound is likely related to the concentration added to cells. Optimizing the concentration may result in lower toxicity. Given the cytotoxic nature of MTT, the assay method must be considered as an endpoint assay. A recent report speculated that formazan crystals contribute to harming cells by puncturing membranes during exocytosis. The observation of extracellular formazan crystals many times the diameter of cells that grow longer over time make it seem unlikely that exocytosis of those large structures was involved. Growing crystals may suggest that marginally soluble formazan accumulates where seed crystals have begun to deposit. Reducing compounds are known to interfere with tetrazolium reduction assays. Chemicals such as ascorbic acid, or sulfhydryl-containing compounds including reduced glutathione, coenzyme A, and dithiothreitol, can reduce tetrazolium salts non-enzymatically and lead to increased absorbance values in assay wells. Culture medium at elevated pH or extended exposure of reagents to direct light also may cause an accelerated spontaneous reduction of tetrazolium salts and result in increased background absorbance values. Suspected chemical interference of test compounds can be confirmed by measuring absorbance values from control wells without cells incubated with culture medium containing MTT and various concentrations of the test compound.