TOMOCUBE

06/03/2026

Congratulations to the research team from the Catholic University of Korea and collaborators on their new publication in Cell Reports Physical Science. 🎉

In this work, the authors introduce a tunable nitrogen–oxygen plasma coating strategy that transiently reverses the surface charge of cell membrane-derived nanovesicles, remodels their protein corona, and significantly enhances systemic doxorubicin delivery and antitumor efficacy.

Using live-cell 3D holotomography imaging combined with fluorescence detection, the team visualized real-time intracellular trafficking, nuclear delivery of doxorubicin, and apoptosis progression. This quantitative phase imaging approach enabled precise monitoring of vesicle internalization kinetics and provided dynamic mechanistic insight into how plasma-induced surface modulation enhances endocytic uptake and therapeutic performance.

A compelling demonstration of how physical surface engineering and advanced holotomography can synergize to optimize next-generation nanomedicine platforms.

https://www.sciencedirect.com/science/article/pii/S2666386425006836

03/03/2026

𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝗱 𝟯𝗗 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝘀𝗵𝗼𝘂𝗹𝗱𝗻’𝘁 𝗺𝗲𝗮𝗻 𝗰𝗼𝗺𝗽𝗹𝗶𝗰𝗮𝘁𝗲𝗱 𝘄𝗼𝗿𝗸𝗳𝗹𝗼𝘄.

The 𝗛𝗧-𝗫𝟭 𝗦𝗲𝗿𝗶𝗲𝘀 is designed for advanced 3D label-free imaging—without adding complexity to your experiment.

🔹 Seamless handling of various biological models
🔹 Compatibility with diverse labware
🔹 Autofocus
🔹 Automatic calibration
🔹Environmental control

From setup to acquisition, everything is designed to reduce friction in your daily workflow—so you can spend more time analyzing data, not adjusting optics.

See how simplified 3D imaging looks in practice.
YouTube: https://www.youtube.com/watch?v=biRrxmXX2g8

23/02/2026

Congratulations to the authors from Mayo Clinic and their collaborators on the publication of this exciting work in Nature Immunology! 🎉

In this study, the team demonstrates that SIRT2-mediated deacetylation of LCK at lysine 228 fine-tunes T cell receptor (TCR) signaling strength, thereby shaping T cell activation, thymic selection, repertoire diversity, and antitumor immunity.

Using , our users were able to visualize and quantitatively analyze cellular morphology and dynamic changes in T cells in a label-free manner, providing high-resolution, 3D insights into how modulation of SIRT2 activity impacts T cell functional states and signaling responsiveness. This advanced imaging approach complemented the molecular and biochemical analyses by enabling precise, non-invasive characterization of cellular phenotypes associated with altered TCR signaling.

Go to the article: https://www.nature.com/articles/s41590-025-02377-3

Phosphorylation-dephosphorylation and ubiquitination tune TCR signaling to avoid self-reactivity. Kim and colleagues show that acetylation also modulates TCR signaling.

16/02/2026

🧧✨ Happy Lunar New Year from Tomocube! ✨🧧

While the calendar year is already underway, the Lunar New Year offers a special moment to pause, reconnect, and look ahead with a fresh perspective.

We’d like to take this opportunity to thank our global community—partners, customers, and collaborators—for the ideas, conversations, and shared progress that continue to shape what we do.

May the months ahead bring steady momentum, inspiring breakthroughs, and plenty of moments worth celebrating, wherever you are in the world. We’re grateful to be on this journey with you. 🍊

09/02/2026

🚀 𝗤𝘂𝗮𝗻𝘁𝗶𝘁𝗮𝘁𝗶𝘃𝗲, 𝗟𝗮𝗯𝗲𝗹-𝗙𝗿𝗲𝗲 𝗢𝗿𝗴𝗮𝗻𝗼𝗶𝗱 𝗗𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝟯𝗗

We’re excited to share a recent preprint from a collaborative study between Tomocube, Inc. and 𝗞𝗔𝗜𝗦𝗧 𝗕𝗠𝗢𝗟, introducing a robust framework for morphology-preserving, quantitative analysis of live dynamics using holotomography.

🔬 What’s the challenge?
- Organoids undergo complex 3D dynamics (expansion, collapse, fusion)
- Fluorescence imaging alone limits long-term, quantitative studies due to labeling, phototoxicity, axial distortion & reconstruction artifacts

💡 What’s new in this work?
- Introduces Morphology-Preserving Holotomography (MP-HT)
- A physics-aware representation that mitigates missing-cone artifacts
- Enables robust 3D segmentation of epithelium & lumen
- Supports morphology-independent RI & dry-mass quantification

📊 Why it matters
- Long-term, label-free tracking of live organoid dynamics
- Quantitative readouts of epithelial–lumen remodeling, dry-mass density, and total dry mass
- Captures biophysical changes difficult to observe with fluorescence

Read more: https://arxiv.org/abs/2512.22486

03/02/2026

📍 LMA2026 — Good Morning from the Symposium Floor

Light Microscopy Australia’s 5th National Symposium is officially underway. We’re excited to be here together with our partner, Scitech, kicking off a full week of conversations around live imaging, advanced light microscopy, and the future of image analysis.

Looking forward to connecting with the community and sharing how label-free, quantitative 3D holotomography complements today’s imaging workflows.

Let the week begin!

02/02/2026

We’re excited to share that Tomocube will be joining SLAS 2026! 🎉

At this year’s show, we’ll be highlighting how HT-X1 Plus supports 𝗵𝗶𝗴𝗵-𝘁𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁, 𝗮𝘂𝘁𝗼𝗺𝗮𝘁𝗶𝗼𝗻-𝗿𝗲𝗮𝗱𝘆 𝗵𝗼𝗹𝗼𝘁𝗼𝗺𝗼𝗴𝗿𝗮𝗽𝗵𝘆—designed for labs that need speed, consistency, and scalable data generation. Our focus is on building an efficient data factory: smart scheduling, reliable auto-focus, and streamlined workflows that help teams acquire large, reproducible datasets with less manual effort.

If you’re working in high-throughput screening, workflow automation, or advanced imaging, come see how semi-automation can make a real difference in day-to-day experiments.

📍 See you at SLAS 2026—let’s talk automation, efficiency, and better data.

30/01/2026

🎉 Congratulations to the Markus Basan Lab on their new Cell publication.

We’re proud to see Tomocube holotomography featured as a quantitative measurement method in the newly published study.

In this work, the authors combined multiple advanced approaches to investigate how cells sense tissue density and mechanical pressure. Holotomography was used to independently quantify cellular biomass density, providing 3D volume reconstruction and refractive-index–based mass measurements that supported the paper's core biophysical conclusions.

This study highlights how holotomography can contribute to:
→ Quantitative measurement of intrinsic cellular properties
→ Label-free analysis of live-cell physical states
→ Complementary validation alongside other high-end biophysical techniques

We’re excited to see holotomography supporting this level of fundamental discovery.

Go to the article: https://www.cell.com/cell/abstract/S0092-8674(25)01253-X

📩 For scientific inquiries or collaboration discussions: info@tomocube.com

Cells in tissues need to know when to start growing and when to stop growing to quickly heal wounds but prevent tumorous overgrowth. Membrane potential allows cells to sense physical forces, including those from neighboring cells, and thereby regulates growth.

28/01/2026

We’re excited to be heading to — Light Microscopy Australia’s 5th National Symposium!

📅 February 2-6, 2026

A full week dedicated to live imaging, advanced techniques, and the future of image analysis.

We’re looking forward to exchanging ideas, discovering new methods, and sharing how label-free, quantitative 3D holotomography fits into the evolving imaging ecosystem.

See you there!

27/01/2026

📢 New Holotomography Video

Discover the inner architecture of the 𝗲𝗺𝗯𝗿𝘆𝗼𝗻𝗶𝗰 𝗺𝗼𝘂𝘀𝗲 𝗰𝗼𝗰𝗵𝗹𝗲𝗮 — without labels, staining, or thin sectioning.

This video showcases label-free 3D imaging of intact embryonic mouse cochlear tissue using holotomography (HT), revealing fine anatomical structures such as hair cell rows, blood vessels, and neural features based purely on intrinsic optical contrast.

Why holotomography for 𝘁𝗶𝘀𝘀𝘂𝗲 𝗶𝗺𝗮𝗴𝗶𝗻𝗴?

🔹 Direct observation of tissue's microstructure
🔹 Reveal structures that are hard to label
🔹 Preserve imaging samples for downstream analysis
🔹 Simplified experimental workflow

YouTube link: https://www.youtube.com/watch?v=w0WQJ535i2g
📩 Learn more about holotomography: https://www.tomocube.com
📧 Contact us: info@tomocube.com

#토모큐브 #달팽이관

22/01/2026

A small moment, but a meaningful one for our team.

We were genuinely moved to receive these pictures from Prof. Chan-gi Pack (Asan Medical Center / Ulsan University College of Medicine), holding framed holotomography images of his own samples, with a bright smile that says more than words.

Prof. Pack has been a valued collaborator and long-time user of our technology. His trust, engagement, and ongoing dialogue with our team represent the kind of partnership we deeply value.

Thank you for the continued collaboration and support. It truly means a lot to us.

16/01/2026

🎉 Congratulations to the authors from The University of Texas MD Anderson Cancer Center on their exciting new study published in Cell Reports Medicine, revealing a previously unrecognized metabolic vulnerability in triple-negative breast cancer (TNBC).

This study reveals a novel 𝗺𝗶𝘁𝗼𝗰𝗵𝗼𝗻𝗱𝗿𝗶𝗮𝗹 𝗾𝘂𝗮𝗹𝗶𝘁𝘆-𝗰𝗼𝗻𝘁𝗿𝗼𝗹 𝗽𝗿𝗼𝗴𝗿𝗮𝗺 𝗶𝗻 𝗧𝗡𝗕𝗖 𝗰𝗲𝗹𝗹𝘀, where damaged mitochondria are cleared through extracellular vesicles—working alongside classical mitophagy to support cancer cell survival. Importantly, disrupting this pathway leads to mitochondrial collapse and cancer cell death, highlighting a promising therapeutic opportunity.

🧠Label-free 3D played a key role in this work, enabling researchers to directly observe mitochondrial remodeling and cellular stress responses over time. By providing quantitative, longitudinal insight into live-cell dynamics—without staining or perturbation—holotomography helped reveal cellular behaviors that are difficult to capture with conventional imaging.

Read the full article: https://doi.org/10.1016/j.xcrm.2025.102478