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26/02/2026

O.A. Listeria Agar

O.A. (Ottaviani & Agosti) Listeria Agar is a chromogenic selective medium used to isolate and differentiate Listeria species, especially Listeria monocytogenes, mainly in food and environmental samples.

๐Ÿ”ฌ Purpose Detection

Used according to ISO 11290 standard for food safety testing.

๐ŸŽจ Colony Appearance (37ยฐC, 24โ€“48 hrs)

L. monocytogenes โ†’ Blue-green colonies with opaque halo
L. innocua โ†’ Blue-green colonies without halo
Most other bacteria โ†’ Inhibited

๐Ÿ“ฆ Available As
Dehydrated powder
Ready-to-use plates
โ„ Storage
Plates: 2โ€“8ยฐC

๐Ÿ‘‰ Used widely in food microbiology labs for rapid presumptive identification of Listeria monocytogenes.

Ready to elevate your testing ?

๐Ÿ“ฉ Drop email for inquires
imransco@gmail.com

๐ŸŒ Website for latest update
Imransco.com

23/02/2026

Reliable determination is the backbone of effective therapy MIC Test Strips deliver consistent gradients and clear endpoints because patient care starts with precise laboratory results.

Ready to elevate your testing ?

๐Ÿ“ฉ Drop email for inquires
imransco@gmail.com

๐ŸŒ Website for latest update
Imransco.com

  Amies Transport Medium with Charcoal is a semisolid, non-nutritive transport medium designed to preserve the viability...
19/02/2026

Amies Transport Medium with Charcoal is a semisolid, non-nutritive transport medium designed to preserve the viability of microorganisms in clinical swab specimens

๐Ÿ”นPurpose

Designed for collection, transport, and preservation of clinical swab specimens for bacterial culture.

Keeps organisms alive without promoting growth until you inoculate them into culture media.

Common specimen types: throat, wound, ge***al, respiratory swabs.

๐Ÿ”นAdvantages

Charcoal improves viability: charcoal adsorbs toxic substances and helps sensitive bacteria survive during transport (e.g., ).

Standardized quality: meets clinical/CLSI transport standards, consistent recovery performance.

๐Ÿ”นDisadvantages

Not suitable for all fastidious organisms: viability for extremely delicate pathogens may still drop, especially after prolonged transport.

Best within ~12โ€“24 h:
recovery of organisms like decreases over time

๐Ÿ”นViability duration
โฑ๏ธ ~24โ€“48 h

In this blessed month of reflection and gratitude   Imran Scientific Company wishes you a month filled with barakah and ...
18/02/2026

In this blessed month of reflection and gratitude Imran Scientific Company wishes you a month filled with barakah and peace ๐Ÿง‘๐Ÿปโ€๐Ÿ”ฌ๐ŸŒ™

The Disc Dispenser โ€” the true heart of every microbiology lab ๐Ÿ’™๐Ÿฅผ
16/02/2026

The Disc Dispenser โ€” the true heart of every microbiology lab ๐Ÿ’™๐Ÿฅผ

ANTIBIOTIC SENSITIVITY TEST (AST) ๐Ÿงซ๐Ÿ’Š๐Ÿงช ๐ŸŒŸ " AST: The Key to Rational Antibiotic Use " ๐ŸŒŸ ๐ŸŒŸ "AST: Right Drug. Right Dose. Ri...
12/02/2026

ANTIBIOTIC SENSITIVITY TEST (AST) ๐Ÿงซ๐Ÿ’Š๐Ÿงช

๐ŸŒŸ " AST: The Key to Rational Antibiotic Use " ๐ŸŒŸ

๐ŸŒŸ "AST: Right Drug. Right Dose. Right Time " ๐ŸŒŸ

โ‰๏ธ What is AST.....?

" Antibiotic Sensitivity Testing is a laboratory method used to determine the susceptibility or resistance of bacteria to antibiotics, guiding effective treatment."

๐Ÿงซ PURPOSE OF AST ๐Ÿงซ

โœ”๏ธ Select the most effective antibiotic
โœ”๏ธ Avoid antibiotic resistance
โœ”๏ธ Guide clinicians for targeted therapy
โœ”๏ธ Monitor emerging drug resistance

๐Ÿ’Š COMMON METHODS OF AST ๐Ÿ’Š

๐Ÿ“‰1๏ธโƒฃ Kirbyโ€“Bauer Disc Diffusion Method ๐Ÿ“‰

๐Ÿ”บ๏ธ Most commonly used method
๐Ÿ”บ๏ธ Antibiotic discs placed on Mueller-Hinton agar
๐Ÿ”บ๏ธ Measures zone of inhibition
๐Ÿ”บ๏ธ Results: Sensitive (S)
Intermediate (I)
Resistant (R)

๐Ÿ“‰2๏ธโƒฃ Broth Dilution Method

๐Ÿ”บ๏ธ Determines Minimum Inhibitory Concentration (MIC)
๐Ÿ”บ๏ธ Can be macro-dilution or micro-dilution

๐Ÿ“‰3๏ธโƒฃ E-Test (Gradient Diffusion)๐Ÿ“‰

๐Ÿ”บ๏ธ Plastic strip with antibiotic gradient
๐Ÿ”บ๏ธ Direct MIC reading where growth intersects strip

๐Ÿ“‰4๏ธโƒฃ Automated Systems๐Ÿ“‰

๐Ÿ”บ๏ธ Example: VITEK, Phoenix
๐Ÿ”บ๏ธ Faster and standardized results

๐Ÿ“Š REPORTING OF RESULT ๐Ÿ“Š

Based on CLSI / EUCAST guidelines
Results expressed as:

๐ŸŸข Sensitive (S)
๐ŸŸก Intermediate (I)
๐Ÿ”ด Resistant (R)

๐Ÿ“Š๐Ÿงช STEP - BY - STEP PROCEDURE OF AST ๐Ÿ“Š๐Ÿงช

๐Ÿงซ 1. Clinical Sample

โฌ‡๏ธ

๐Ÿงช 2. Isolation of Bacteria
(Pure culture on culture media)

โฌ‡๏ธ

๐Ÿงช 3. Preparation of Inoculum
(Adjust to 0.5 McFarland standard)
โฌ‡๏ธ

๐Ÿงซ 4. Inoculation on Agar Plate
(Muellerโ€“Hinton agar lawn culture)

โฌ‡๏ธ

๐Ÿ’Š 5. Placement of Antibiotic Discs
(Using sterile forceps/dispenser)

โฌ‡๏ธ

๐Ÿ”ฅ 6. Incubation
(37ยฐC for 16โ€“18 hours)

โฌ‡๏ธ

๐Ÿ“ 7. Measurement of Zone of Inhibition
(Measure diameter in mm)

โฌ‡๏ธ

๐Ÿ“Š 8. Interpretation of Results
(Sensitive / Intermediate / Resistant)

โฌ‡๏ธ

โœ…๏ธ 9. Reporting
(As per CLSI / EUCAST guidelines)

๐ŸŸข Sensitive (S)
๐ŸŸก Intermediate (I)
๐Ÿ”ด Resistant (R)

๐ŸฅผCLINICAL IMPORTANCE ๐Ÿฅผ

๐Ÿ”ท๏ธ Prevents misuse of antibiotics
๐Ÿ”ท๏ธ Reduces multidrug-resistant organisms (MDROs)
๐Ÿ”ท๏ธ Essential for infection control & surveillance
๐Ÿ”ท๏ธ Supports antibiotic stewardship programs

๐ŸŸข Pure culture is essential
๐ŸŸข Standard inoculum (0.5 McFarland)
๐ŸŸข Correct incubation time & temperature
๐ŸŸข Accurate measurement of inhibition zones

๐Ÿ’ซ " AST is a cornerstone of clinical microbiology, ensuring safe, effective, and rational antibiotic therapy...." ๐Ÿ’ซ


It was a privilege to meet Mr. Fabio Brocco,CEO of   at the   and learn about their latest innovations in   and   soluti...
11/02/2026

It was a privilege to meet Mr. Fabio Brocco,CEO of at the and learn about their latest innovations in and solutions.

  Microbiology Culture Media ๐Ÿฆ  CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) is a differential culture medium m...
11/02/2026

Microbiology Culture Media ๐Ÿฆ 



CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) is a differential culture medium mainly used for isolating and identifying bacteria from urine samples. It supports the growth of urinary pathogens and helps differentiate lactose-fermenting bacteria from non-lactose fermenters while preventing the swarming of Proteus species.



PPLO Broth (Pleuropneumonia-Like Organism Broth) is a specialized liquid culture medium used for the cultivation of Mycoplasma species. It contains nutrients required by fastidious organisms and is commonly used in clinical and research laboratories.



Nutrient Broth is a general-purpose liquid medium used for the cultivation of a wide range of non-fastidious bacteria. It is widely used for routine laboratory work, bacterial growth studies, and maintaining bacterial cultures.

07/02/2026
07/02/2026
Nutrient Agar ISO 16266 โ€“ LiofilchemยฎOpt for precision and reliability with ISO 16266 Standard Nutrient Agar, ideal for ...
04/02/2026

Nutrient Agar ISO 16266 โ€“ Liofilchemยฎ

Opt for precision and reliability with ISO 16266 Standard Nutrient Agar, ideal for microbiological analysis and quality control.

โœ… Complies with international standards
โœ… High performance for bacterial culture
โœ… Reliable and reproducible results

Ready to elevate your testing ?

๐Ÿ“ฉ Drop email for inquires
imransco@gmail.com

๐ŸŒ Website for latest update
Imransco.com

  Disc Dispenser is a laboratory microbiology tool used mainly in antimicrobial susceptibility testing (AST).๐–๐ก๐ฒ ๐ข๐ฌ ๐ข๐ญ ๐ฎ...
29/01/2026

Disc Dispenser is a laboratory microbiology tool used mainly in antimicrobial susceptibility testing (AST).

๐–๐ก๐ฒ ๐ข๐ฌ ๐ข๐ญ ๐ฎ๐ฌ๐ž๐?

๐Ÿ”นTo save time
๐Ÿ”นTo avoid touching discs by hand
๐Ÿ”นTo ensure equal distance between antibiotic discs
๐Ÿ”นTo give accurate and reproducible results

๐‡๐จ๐ฐ ๐ข๐ญ ๐ฐ๐จ๐ซ๐ค๐ฌ?

๐Ÿ”นPrepare agar plate with bacterial culture
๐Ÿ”นLoad antibiotic discs into the dispenser
๐Ÿ”นPlace dispenser on agar plate
๐Ÿ”นPress the handle/button
Discs are placed

๐€๐๐ฏ๐š๐ง๐ญ๐š๐ ๐ž๐ฌ

โœ… Fast and efficient
โœ… Accurate disc positioning
โœ… Reduces human error
โœ… Improves test reliability

๐‘๐ž๐ฅ๐š๐ญ๐ž๐ ๐“๐ž๐ฌ๐ญ

๐Ÿ”นKirbyโ€“Bauer Disc Diffusion Method
๐Ÿ”นBased on CLSI / EUCAST guidelines
Complete Information:
http://www.liofilchem.net/login/pd/pi/91200_PI.pdf

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Karachi
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