Musaji Adam & Sons

29/11/2025

🔬 Why Do We Draw 7–10 mL in a Signal Blood Culture oxoid ?
The Hidden Science Behind the Number

In every microbiology lab, one golden rule never changes:
“Collect 7–10 mL of blood per culture bottle"

But have you ever wondered why this specific volume matters so much?

Here’s the science behind it:

👉 1️⃣ Bacteremia is usually low-level
In many septic patients, bacterial load can be as low as 1 CFU/mL.
Drawing only 2–3 mL dramatically increases the risk of missing the pathogen.

👉 2️⃣ More volume = Higher detection rate
Evidence shows that each additional milliliter improves detection.
The sweet spot? 7–10 mL — the optimal balance of sensitivity and practicality.

👉 3️⃣ Bottle chemistry is designed for that volume
Blood culture bottles contain:
• Anticoagulants
• Resins / additives to neutralize immune factors
Their chemistry works best when the bottle receives 7–10 mL, not less or more.

👉 4️⃣ Reduces false negatives
Low sample volume = one of the most common causes of falsely negative cultures
(especially in septicemia).

👉 5️⃣ Global guidelines agree
CLSI, CDC, ESC all recommend:
✔ Ideal volume: 7–10 mL per bottle

🧪 Accurate blood culture = Better antimicrobial stewardship (AMS)
Better AMS = Reduced antimicrobial resistance (AMR)

Because every correct detection leads to smarter antibiotic decisions.

💡 Consistency in blood volume isn’t just a protocol… it’s a clinical impact.

PC: Ashraf Frag

28/11/2025

Event announcement 📣

27/11/2025

Product Announcement

Introducing a Groundbreaking Innovation in Beverage Spoilage Quality Testing

We are thrilled to announce the launch of the Thermo Scientific SureTect Beverage Spoilage Multiplex qPCR Assay, a game-changing solution developed in collaboration with beverage industry leaders at CocaCola Europacific Partners.

Microbial spoilage continues to pose a significant challenge to beverage manufacturers worldwide, with traditional testing methods often falling short. This new all-in-one kit is set to revolutionize the industry.

Key features of this innovative solution include:

- Speed: Accelerate your testing process with results up to 4 days faster than conventional methods
- Efficiency: Streamline your workflow with reduced hands-on time and improved productivity
- Sustainability: Minimize your environmental footprint with reduced plastic waste and energy consumption
- Brand Protection: Fortify your brand reputation with reduced risk of recalls and product scraps

With this cutting-edge solution, we are empowering beverage manufacturers to safeguard quality, optimize workflows, and drive growth.

Discover how we are shaping the future of beverage quality testing: http://spr.ly/604379HZf
OR drop an email to info@musajisons.com for further assistance.

New Multiplex qPCR Assay developed in collaboration with Coca-Cola Europacific Partners delivers faster, broader detection to help protect product quality and brand reputation To support the quality of beverage products, Thermo Fisher Scientific today introduced the Thermo Scientific™ SureTect™ ...

20/11/2025

𝐖𝐡𝐲 𝟏𝟎𝐠/𝟏𝟎𝐦𝐋 𝐢𝐬 𝐰𝐞𝐢𝐠𝐡𝐢𝐧𝐠 𝐟𝐨𝐫 𝐌𝐋𝐓 𝐚𝐧𝐚𝐥𝐲𝐬𝐢𝐬 (𝐑𝐚𝐰 𝐦𝐚𝐭𝐞𝐫𝐢𝐚𝐥) ?

𝐌𝐢𝐧𝐢𝐦𝐮𝐦 𝐫𝐞𝐪𝐮𝐢𝐫𝐞𝐦𝐞𝐧𝐭:
🔹Every batch size has been calculated based on the required concentration of active substances.
🔹As per guideline (USP 61,62), the amount of raw material tested shall be 1% of the total active substances for extremely small batch (less than 1000g or 1000mL).
🔹For example, if the batch size 1000g means, the analysis should be performed with minimum 10g sample (1%). So 10g is a minimum required range to execute a microbial limit test.
🔹Some exceptional cases a lesser amount is prescribed, but need to justified and authorized (Eg: Clinical trials).
𝐒𝐭𝐚𝐧𝐝𝐚𝐫𝐝𝐢𝐳𝐚𝐭𝐢𝐨𝐧:
🔹For MLT, the results have encoded in "gram" or "mL" i.e

19/11/2025

Musaji Adam & Sons | AMR Awareness Week 2025 – Pakistan

Strengthening the fight against antimicrobial resistance

We are proud to be part of Awareness Week 2025, standing alongside leading national and international organizations — including DUHS, FAO, the Ministry of Food & Drug Safety, and several esteemed universities and institutes — in raising the global voice against antimicrobial resistance.

Throughout the week, our team:

✔️ Showcased cutting-edge AMR solutions
✔️ Engaged with researchers, faculty members, and students
✔️ Promoted responsible practices to combat one of the world’s most urgent health threats

At Musaji Adam & Sons, our commitment to advancing science remains stronger than ever. With over six decades of service, we continue to drive innovation and support the scientific community —Serving Science Since 1960.

📧 info@musajisons.com
🌐 www.musajisons.com

13/11/2025

Introducing the IMMY CrAg® LFA – Rapid Cryptococcal Antigen Detection

Proud to share one of the most reliable, fast, and user-friendly diagnostic tools now available through Musaji Adam & Sons.

The CrAg® Lateral Flow Assay (LFA) is an advanced immunochromatographic test designed for the qualitative and semi-quantitative detection of Cryptococcus species complex (C. neoformans & C. gattii) in serum and CSF.

- Why it matters
Cryptococcosis remains a critical global health challenge, especially among immunocompromised patients. Early diagnosis can dramatically improve outcomes — and this assay offers a powerful solution.

- Key Features
✔️ Results in just 10 minutes
✔️ Highly sensitive & specific
✔️ No pretreatment required
✔️ Easy 5-step workflow
✔️ CE marked | IVD compliant

Designed for efficient, accurate, and accessible diagnostics in any laboratory setting.

At Musaji Adam & Sons, we remain committed to serving healthcare professionals with reliable diagnostic solutions since 1960.

For inquiries or orders:
📧 info@musajisons.com
🌐 www.musajisons.com

10/11/2025

How to Use Primer-BLAST (NCBI)

1️⃣ Input PCR Template
Enter an Accession number, GI, or FASTA sequence, or upload a FASTA file.
Optionally define the range or insert your own forward/reverse primers.

2️⃣ Set Primer Parameters
Choose whether to design new primers or use existing ones.
Define PCR product size (e.g., 70–1000 bp).
Adjust melting temperature (Tm), typically 57–63 °C.
Optionally, prefer primers located near the 3′ end.

3️⃣ Exon/Intron Settings (for mRNA templates)
Select exon–intron junction preferences.
Define minimum/maximum match requirements across junctions.

4️⃣ Check Primer Specificity
Enable specificity check to avoid off-target binding.
Select a database (e.g., RefSeq mRNA).
Specify the organism.
Set stringency rules: mismatches allowed, target size, splice variant options.

5️⃣ Generate Primers
Click Get Primers.
Review the output list: primer sequences, melting temperatures, locations, E Value, and BLAST validation.

PC: Ibrahim Atef Ibrahim

09/11/2025

Why Are Buffers Essential in HPLC Analysis?

In High-Performance Liquid Chromatography (HPLC), your mobile phase isn’t just a solvent — it’s the heart of your separation. And within it, buffers play a silent yet powerful role.

Let’s break down why buffers matter so much 👇

🔹 1. pH Control
Buffers help maintain a stable pH, which is crucial because:
The ionization state of analytes depends on pH — affecting polarity and retention.
Many stationary phases are pH-sensitive; stability ensures longer column life and consistent results.

🔹 2. Improved Peak Shape & Resolution
A controlled pH means:
Reduced peak tailing
Better column efficiency
Sharper resolution for closely eluting analytes

🔹 3. Reproducibility & Reliability
Consistent buffer conditions = reproducible results ✅
This is vital for:
Method development & validation
Accurate quantitation
Meeting regulatory standards in pharma & analytical labs

🔹 4. Column Protection
Buffers protect against:
pH-related column degradation
Damage from excessive analyte ionization

🧪 Common Buffers in HPLC:
1) Phosphate buffer:
2) Acetate buffer:
3) Formate buffer:
4) Ammonium buffer:

PC: HARSHAL PATIL

08/11/2025

Bridging Academia & Industry | Health Technology Collaboration

We’re thrilled to announce a game-changing Memorandum of Understanding between , and — a strategic partnership designed to ignite innovation between academia and industry!

This collaboration paves the way for transformative initiatives, including:
• High-impact research projects
• Exclusive student internships
• Joint education and professional training programs

With a strong focus on Health Technology, this MoU marks a pivotal step toward building a future where academic excellence seamlessly translates into real-world commercial and societal impact.

, we have always believed in empowering the next generation by fostering stronger connections between students, research, and industry.

Here’s to innovation, collaboration, and meaningful progress — together!

24/10/2025

Antimicrobial Resistance (AMR) is escalating fast in Pakistan. Rapid detection is essential to contain its spread and guide the right therapy.

. . .

Detect the “Big Five” carbapenemases in just 15 minutes!

The -Test by is a rapid lateral-flow test that identifies KPC, OXA-48, VIM, IMP, and NDMdirectly from a single colony — no equipment, no delays.

✅ Fast – results in ≤ 15 min
✅ Accurate – detects & differentiates all 5 major enzymes
✅ Easy – colony → buffer → cassette → result
✅ Ideal for infection-control & AMR surveillance
📦 Compact kit | 🌡️ Room-temperature storage | FDA-cleared and CE Marked
✅ Can be performed directly from positive blood cultures — up to 1–2 days faster than conventional methods

Empowering labs in Pakistan to detect resistance early, protect patients, and control outbreaks effectively.

Learn more: https://lnkd.in/dTXq3hbC

22/10/2025

Join Us at 2025!

📅 23–25 October 2025
📍 Karachi Expo Center, Pakistan

We’re excited to announce that & Sons will be exhibiting at Health Asia — International Exhibition & Conferences, showcasing our latest innovations in Clinical Diagnostics, Microbiology, and Molecular Solutions.

Stop by our Booth #24–26 to explore our range of products, meet our team, and discover how we’ve been Serving Science since 1960.

📧 info@musajisons.com
🌐 www.musajisons.com

21/10/2025

We’re Across Pakistan! | Join Adam & Sons

Looking for a rewarding career in the healthcare and diagnostics industry?

We’re offering multiple opportunities for Sales & Marketing Executives in , , and

If you have a background in Medical Laboratory Technology (MLT) or Microbiology, and a passion for connecting with healthcare professionals — this is your chance to grow with a company that’s been Serving Science Since 1960.

What We’re Looking For
-Bachelor’s in MLT or Microbiology (Mandatory)
-Minimum 2 years’ experience in healthcare sales or customer service (Hospitals, Labs, Diagnostics)
-Strong communication, negotiation, and interpersonal skills
-Self-motivated, goal-driven, and a strong team player

What We Offer
-Biannual Bonus (twice a year)
-Quarterly Product Incentives
-Annual Target Bonus
-Comprehensive Health Insurance (individual & family)

📩 Apply Now: info@musajisons.com

📍 Locations: Lahore | Islamabad | Karachi

Spread the word among your college networks — help someone start their next big career move!