03/08/2024
PANADOL ( TYLENOL/PARACETAMOL) DOESN'T AFFECT THE RAPID DIAGNOSTIC TEST RESULTS FOR MALARIA.
The malaria Pf/Pan Ag Rapid Test is a rapid lateral flow chromatographic immunoassay for simultaneous detection and differention of HRP-II( Histadine -Rich Protein II ) spefic to plasmodium falciparum and pLDH ( Plasmodium lactate dehydrogenase) specific to Plasmodium species ( Pan) in human blood specimen as an aid in Diagnosis of malaria infection.
PRINCIPLE
The malaria Pf/Pan Ag Rapid Test contains a membrane strip, which is precoated with mouse monoclonal antibodies specific to HRP-II of P. falciparum on test line PF(T2) region and with mouse monoclonal antibodies specific to lactate dehydrogenase of plasmodium species ( P.falciparum, P. Vivax, P.malariae and P.ovale ) on test line Pan(T1) region respectively. A conjugate pad is dispansed with mixture of mouse monoclonal antibodies specific to HRP-II OF P.f and mouse monoclonal antibodies specific to pLDH of pan-collidal gold. During the assay, an adequate volume of blood specimen is dispensed into the sample well (S) of the test cassette, a lysis buffer is added to the buffer well (B). The buffer contains a detergent that lyses the red blood cells and releases various antigens, which migrate by capillary action across the strip held in the cassette. PanLDH if presents in the specimen will bind to the Pan-LDH-gold conjugates. The immunocomplex is then captured on the membrane by the precoated anti-Pan-LDH antibody, forming a burgundy colored Pan(T1) band, indicating a pan positive Test result. Alternatively, pHRP-II if presents in the specimen will bind to the pHRP-II -gold conjugates. The Immunocomplex is then captured on the membrane by precoated anti-pHRP-II Antibodies, forming a burgundy colored Pf(T2) band indicating a Pf positive test result.
Absence of any T bands suggests a negative results. The test contains an internal control (C band ) which should exhibit a burgundy colored band of the immunocomplex of goat anti-mouse IgG/mouse IgG ( anti-Pan-LDH and anti-pHRP-II) gold conjugates regardless of the color development of any of the T Bands. Otherwise, the test result is invalid and the specimen must be retested with another device.
Considering the MOA for paracetamol which has central analgesic effect that is mediated through activation of descending serotonergic pathways. Its also a weak inhibitor of of Prostaglandin synthesis of COX-1 and COX-2 in broken cells, but by contrast, therapeutic concentrations of paracetamol inhibit PG synthesis in intact cells in vitro when the level of the substrate arachdonic acid are low ( less than about 5mumol/L. ) At no point does the effect of paracetamol affect the production of HRP-II.
It's worthy knowing that even when u take the actual Antimalarial drugs prior to Testing the Random Diagnostic Test it will still give a positive result in indeed you have the Infection. The furthermore the RDT remains positive for 7 (2-20dys ) post Treatment.
This is just basic human physiology and applied Pharmacology and Therapeutics. We are no longer treating malaria clinically and never should be tempted to do. If u ain't satisfied with RDT results please feel free to order for MPS ( Malaria Parasite Smear ) for proper Diagnosis. Otherwise malaria can be fetal and please don't take chances.
Tez Fortified.
